| 质粒名称: | pGL3 2 kb prom. CD274 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 107003 |
| 存储实验室: | Julian Downward |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3 |
| 载体出品公司: | Promega |
| 载体抗性: | 氨苄青霉素 |
| 载体类型: | 哺乳动物表达，荧光素酶 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | CD274,PD-L1, B7-H1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 2203 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | MluI（销毁后未知） |
| 3' 克隆位点: | XhoI（销毁后未知） |
| 5' 测序引物 | RVprimer3 |
| 3' 测序引物 | TCTTTATGTTTTTGGCGTC |
|
文献引用方法 |
|
| 材料和方法部分: | pGL3 2 kb prom. CD274 was a gift from Julian Downward (Addgene plasmid # 107003 ; http://n2t.net/addgene:107003 ; RRID:Addgene_107003) |
| 参考文献部分: | Oncogenic RAS Signaling Promotes Tumor Immunoresistance by Stabilizing PD-L1 mRNA. Coelho MA, de Carne Trecesson S, Rana S, Zecchin D, Moore C, Molina-Arcas M, East P, Spencer-Dene B, Nye E, Barnouin K, Snijders AP, Lai WS, Blackshear PJ, Downward J. Immunity. 2017 Dec 19;47(6):1083-1099.e6. doi: 10.1016/j.immuni.2017.11.016. Epub 2017 Dec 12. 10.1016/j.immuni.2017.11.016 PubMed 29246442 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGL3 2 kb prom. CD274 7010 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Modified Luciferase expression vector.
ACCESSION .
VERSION .
KEYWORDS pGL3 2 kb prom. CD274
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7010)
AUTHORS Coelho MA, de Carne Trecesson S, Rana S, Zecchin D, Moore C,
Molina-Arcas M, East P, Spencer-Dene B, Nye E, Barnouin K, Snijders
AP, Lai WS, Blackshear PJ, Downward J
TITLE Oncogenic RAS Signaling Promotes Tumor Immunoresistance by
Stabilizing PD-L1 mRNA.
JOURNAL Immunity. 2017 Dec 19;47(6):1083-1099.e6. doi:
10.1016/j.immuni.2017.11.016. Epub 2017 Dec 12.
PUBMED 29246442
REFERENCE 2 (bases 1 to 7010)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7010
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(81..100)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 291..312
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 580..628
/note="synthetic polyadenylation signal"
misc_feature 642..733
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 682..701
/label=RVprimer3
/note="pGL3 vector, forward primer"
regulatory 3014..3023
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3020..4672
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(3086..3104)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
polyA_signal 4713..4834
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4724..4743)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 4778..4797
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(5082..5099)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5253..5841)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5333..5352)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6012..6872)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6635..6654
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6873..6977)
/gene="bla"
/label=AmpR promoter
rep_origin 7004..449
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
61 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
121 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
181 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
241 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
301 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
361 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
421 ttttaacaaa atattaacgc ttacaatttg ccattcgcca ttcaggctgc gcaactgttg
481 ggaagggcga tcggtgcggg cctcttcgct attacgccag cccaagctac catgataagt
541 aagtaatatt aaggtacggg aggtacttgg agcggccgca ataaaatatc tttattttca
601 ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa
661 aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc
721 agaacatttc tctatcgata ggtaccgagc tcttacgcgt gagacactct gagaaacagc
781 acattggcaa tgatgaattt atatgtctaa aaatgaatag agtagatgtt acatgaatag
841 gaagtggtgg tattcaagat gaccatagta tctagctctt cagccctggc tccccactgc
901 tcttctccca tctcagcact tctccatcta tttcgtccaa ataaaaaaat tcacacagag
961 tttcaggact taacccccca ctcattaacc atctgttttg ctttacatat ttttctgagg
1021 taataaaatt tctctttttc taaacacagc ctgtttttca atctccgggt agttgatcaa
1081 ttgtatggga aaatgaatgg ctgaagggta gaaacaggtg ggaaagatga acaaaaacac
1141 gaatcctcac attactaata cgcaaatcac tgagcagcaa gctgagcaaa taccctcaat
1201 tcccatcagc aactttagag aaaggcaaat tccgtttgcc tcattgatca tttaggtaga
1261 ccctgaacac tgctttcata aaacaaaaac aaaataccca tccccagttt aaaaaattat
1321 tcatagatca tccaggccat ctaggaggat atgatttaat cctggctact tggtaaatta
1381 tttgcccaag ttaactcagc tagttagtgg tagatggctc tgaagccagt tgtttttttt
1441 tgttttgttt tttgcagacc tcaagagtca tgatgaacta gcagatcata aagtttatgc
1501 cctgggtctt gaccattttt agaaaaataa aacattaaat gaaaatatca gagggcattg
1561 cagatagtag atctaagtat tttttcatga aacttgttgt acatgtgtgt gtcatacaca
1621 gactatatat atgcagtacc tgtaaactgt attgccacat aatgtctata ttttcctaga
1681 ggtcacagtc accaaagttg ggaagtcacc caacttcggg aactttggga agtcacccaa
1741 acttacagtc accaaaattg ctctattcta ctatgtgacc tcaaaagtga tttgaaagaa
1801 ggaacatctg agctgggccc aaaccctatt gcaattttat tggggccaaa gagaactcca
1861 tgctcctgcc aaatcaaggc agtgtcagcc tcaataattt cccagataaa aataaaaatc
1921 tgtgatacaa tcagaatgtg aaaattctta ttttggaagc aaatgtcata accaatgcaa
1981 gggctatctc aatattcatt cattatgcag tattttgaac tgcagttgaa atgaataaga
2041 aggaaaggca aacaacgaag agtccaattt ctcaatttag aaaaagagaa aaaaaagaaa
2101 agggagcaca caggcacggt ggctcaagcc tgtaatatca gcactttggc ggatcacttg
2161 aggtcaagga gttcgagaaa agagagcacc tagaagttca gcgcgggata atacttaagt
2221 aaattatgac accatcgtct gtcatcttgg gcccattcac taacccaaag ctttcaaaag
2281 ggctttctta accctcacct agaataggct tccgcagcct taatccttag ggtggcagaa
2341 tatcagggac cctgagcatt cttaaaagat gtagctcggg atgggaagtt cttttaatga
2401 caaagcaaat gaagtttcat tatgtcgagg aactttgagg aagtcacaga atccacgatt
2461 taaaaatata tttcctatta tacacccata cacacacaca cacacctact ttctagaata
2521 aaaaccaaag ccatatgggt ctgctgctga ctttttatat gttgtagagt tatatcaagt
2581 tatgtcaaga tgttcagtca ccttgaagag gcttttatca gaaaggggga cgcctttctg
2641 ataaaggtta aggggtaacc ttaagctctt acccctctga aggtaaaatc aaggtgcgtt
2701 cagatgttgg cttgttgtaa atttcttttt ttattaataa catactaaat gtggatttgc
2761 tttaatcttc gaaactcttc ccggtgaaaa tctcatttac aagaaaactg gactgacatg
2821 tttcactttc tgtttcattt ctatacacag ctttattcct aggacaccaa cactagatac
2881 ctaaactgaa agcttccgcc gatttcaccg aaggtcagga aagtccaacg cccggcaaac
2941 tggatttgct gccttgggca gagctcgaga tctgcgatct aagtaagctt ggcattccgg
3001 tactgttggt aaagccacca tggaagacgc caaaaacata aagaaaggcc cggcgccatt
3061 ctatccgctg gaagatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc
3121 cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtggaca tcacttacgc
3181 tgagtacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac
3241 aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg
3301 cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt
3361 gctcaacagt atgggcattt cgcagcctac cgtggtgttc gtttccaaaa aggggttgca
3421 aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa aaaattatta tcatggattc
3481 taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc
3541 cggttttaat gaatacgatt ttgtgccaga gtccttcgat agggacaaga caattgcact
3601 gatcatgaac tcctctggat ctactggtct gcctaaaggt gtcgctctgc ctcatagaac
3661 tgcctgcgtg agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga
3721 tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg
3781 atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgtttct
3841 gaggagcctt caggattaca agattcaaag tgcgctgctg gtgccaaccc tattctcctt
3901 cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc
3961 tggtggcgct cccctctcta aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc
4021 aggtatcagg caaggatatg ggctcactga gactacatca gctattctga ttacacccga
4081 gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt
4141 ggatctggat accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg
4201 tcctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa
4261 ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat
4321 cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc ccgctgaatt
4381 ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag gtcttcccga
4441 cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac
4501 ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg
4561 aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa
4621 aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgt aattctagag
4681 tcggggcggc cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac
4741 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
4801 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
4861 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
4921 tggtaaaatc gataaggatc cgtcgaccga tgcccttgag agccttcaac ccagtcagct
4981 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
5041 tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac tgactcgctg
5101 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
5161 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
5221 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
5281 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
5341 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
5401 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
5461 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
5521 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
5581 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
5641 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
5701 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
5761 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
5821 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
5881 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
5941 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
6001 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
6061 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
6121 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
6181 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
6241 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
6301 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
6361 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
6421 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
6481 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
6541 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
6601 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
6661 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
6721 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
6781 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
6841 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
6901 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
6961 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc
//
